Food and Chemical Toxicology. 113:322-327.
Rats were given the ADI (acceptable daily intake) amount of Tartrazine (Yellow 5).
- Their body weight was lower.
- The brain protein levels in all their brain sub-regions decreased.
- Lipid peroxide levels in all their brain sub-regions increased.
- Glutathione-S-transferase activity in all brain sub-regions decreased.
- Glutathione reductase activity in some areas of the brain decreased.
- Glutathione peroxidase activity in all brain sub-regions increased.
- MDA levels in all brain sub-regions increased, indicating damage.
RESULTS:
- Sulfanilic acid (a breakdown product of Yellow 5) can cross the blood-brain-barrier.
- Yellow 5 caused severe oxidative stress.
CONCLUSION: The authors concluded that ADI levels are not safe levels.
